Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 29;214(5):603–617. doi: 10.1083/jcb.201603077

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Mason et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Constitutively active RhoA is insufficient for apical constriction. (A) Apical myosin depends on RhoA activity. Images from live embryos expressing myosin::GFP (sqh::GFP) and membrane::RFP (Gap43::mCherry) and injected with buffer (PBS, Control) or the C3 exoenzyme immediately before gastrulation. (B) Apical ROCK localization depends on RhoA activity. Images from live embryos expressing GFP::ROCK and Membrane::RFP injected with solvent or C3 immediately before gastrulation. (C) Constitutively active RhoA (CA-RhoA) disrupts proper myosin organization in ventral furrow cells. Image of a live embryo expressing Myosin::GFP and Membrane::RFP that has been injected at one pole (bottom) with mRNA encoding CA-RhoA (G14V). Time-lapse images (right) show myosin accumulating abnormally at junctions (arrows), specifically in cells proximal to injection. (D) Quantification of total apical myosin intensity in embryo in C injected with CA-RhoA mRNA (n = 47 cells proximal, 44 cells distal from two embryos; error bars represent ±SD). Note that myosin intensity is slightly higher close to the injection site (proximal). a.u., arbitrary units. (E) Quantification of myosin intensity in the middle of the apical surface (medioapical) relative to the junctions. Cells proximal to CA-RhoA injection have a lower medioapical-to-junctional ratio (n = 12 cells proximal, 12 cells distal; **, P < 0.01, unpaired t test; error bars represent ±SD). (F) Apical constriction is impaired in cells proximal to CA-RhoA injection (n = 47 cells proximal, 44 cells distal; error bars represent ±SD). Bars, 5 µm.