Fig. 5.
Visceral adipose tissue pathology in energy dense (ED) diet-fed mice. (A) Representative photomicrographs of visceral adipose tissue (VAT) from standard (SD; n=22) and ED (n=23) diet-fed mice after 30 weeks. (B) Mean adipocyte size (μm2) was significantly greater in VAT from mice fed an ED diet, compared with those fed a SD diet. (C) Concentrations (pg ml−1) of the inflammatory cytokines monocyte chemotactic protein 1 (MCP-1) and interleukin (IL)-6 were markedly higher in VAT of mice fed an ED diet for 30 weeks, compared with SD diet-fed mice. Consistent with this, comparison of equivalent masses (100 mg) of VAT from SD (n=16) and an ED (n=15) diet-fed mice demonstrated significant differences in the frequencies of (D) PMN, (E) macrophages, (F) NK cells, (G) B and T cells and (H) Thelper (Th) and Tcytotoxic (Tc) cells within the stromal vascular fraction. Data are mean±95% c.i. Significant differences were analysed by Student's t-test with Welch's correction and indicated as **P≤0.01, ***P≤0.001, ****P≤0.0001.