Fig. 1.

Shh associates with the multivesicular bodies in growth cones of P2 rat hippocampus and cerebellum. (A) Validation of the specificity of Shh 5E1 antibody. HEK293 cells were transfected with a full-length Shh construct (ShhFL), an N-terminal Shh fragment construct (ShhN), or a control vector. The cell lysates were analyzed by immunoblot using Shh 5E1 antibody. (B) In an axon terminal of the hippocampus, Shh immunogold particles are found in a multivesicular body (mvb). (C) In an axon terminal of the cerebellum, Shh immunogold particles are also found in an mvb. *, postsynaptic density. Scale bars: 100 nm.