Figure 7.

Subunit c of mitochondrial ATP synthase accumulate in lysosomal structures of podocytes as in CD-deficient neurons. (A) Immunohistochemical staining showed subunit c of mitochondrial ATP synthase accumulated in 10-month-old CD^pdKO mouse podocytes. (B and C) Immunoelectron microscopy indicated that, in podocytes from CD^pdKO mice, the labeling for subunit c was associated with membrane-bound structures containing GRODs. Immunocytochemical staining of subunit c of mitochondrial ATP synthase in podocytes from CD^pdKO mice and their CD^Ctrl littermates at 10 months using the cryothin section immunogold method is shown. (B, top panel and C, upper panel) Subunit c gold particles label only the mitochondrial inner membrane in the CD^Ctrl littermates (arrowheads), whereas (B, middle panel and C, lower panel) they are associated with both the inner membrane of intact mitochondria (arrowhead) and the membrane-bound compartments with dense materials (GRODs; arrows) in the CD^pdKO mice.