Figure 8.

The accumulation of podocin in 10-month-old CD^pdKO mouse podocyte cell bodies is particularly colocalized with Lamp1 and Rab7. (A and B) Immunofluorescence staining showed the localization of podocin in the GBM in CD^Ctrl littermates. By contrast, podocin was localized in both the GBM and podocyte cell bodies in CD^pdKO mice. The late endosomal marker Rab7 was increased in CD^pdKO mouse podocytes compared with CD^Ctrl littermate podocytes. Podocin localized in podocyte cell bodies was particularly colocalized with (A) Lamp1 and (B) Rab7 in CD^pdKO mice. (C) For immunohistochemical staining, we detected a strong accumulation of podocin in CD^pdKO mouse podocyte cell bodies. Immunoelectron microscopy using paraffin–embedded tissue sections indicated that the granular structures (GRODs) that accumulate in the podocyte cell bodies of CD^pdKO mouse glomeruli are autophagosomes/autolysosomes containing degraded podocin.