Hhep25 administration in mice reduces endogenous plasma hepcidin-1, hepatic Hamp1 mRNA, and renal ferroportin protein expression. Relative plasma levels of (A) endogenous hepcidin-1, (B) hepatic Hamp1 mRNA expression, and (C) renal ferroportin protein expression in controls and 1 and 24 hours after hhep25 administration. Original Western blots are shown in C, upper panel, and their quantification is shown in C, lower panel. *P<0.05 compared with baseline or control by one-way ANOVA with Bonferroni multiple comparisons test.