Figure 7.

SGK-1 mediates exercise–induced muscle hypertrophy. In WT or SGK-1-KO mice, treadmill-running exercise was performed for 2 weeks after CKD was created. (A and B) The (A) TA and (B) gastrocnemius muscles were collected and weighted (n=6 pairs of mice; P<0.05 versus no exercise mice). (C) Myofiber areas were measured, and their distribution was calculated. (D) Western blot analysis showed the exercise-induced changes in the indicated proteins from WT and SGK-1-KO mice with CKD. (E) Atrogin1 and MuRF1 were analyzed by RT-PCR in TA muscles (n=6). CSA, cross-sectional area; CTL, control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS, non-significant.