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Western blot analysis of trypsin digestion products. Nine IgA1 mAbs were submitted to 10% SDS-PAGE after various digestion times (0–10 hours) by trypsin. Western blot was performed to reveal IgA determinants using HRP–linked goat anti–human IgA. (Left panel) mAb2, mAb4, mAb7, mAb8, mAb9, and mAb10 yield no IgA deposits. (Right panel) mAb6, mAb1, and mAb5 yield deposits.
