Figure 12.

Role of mAb V regions in kidney deposition. IgA1 mAb6 or its IgG1 switched variant was injected in immunodeficient mice 2 hours before euthanasia. IgA1 and IgG1 were revealed on kidney sections with FITC-labeled (green) antisera against either (upper panel) human IgA or (lower panel) IgG. Staining was also with AlexaFluor568 anti–mouse podocin (red). Injection of saline provided a negative control; α1KI and CH1− mice provided controls for the glomerular deposition of human IgA and γ-chains, respectively. Pictures are representative of hundreds of glomeruli observed in kidney sections (two or more mice for each condition). Ab, antibody; Ctrl+, control; i.v., intravenous.