Figure 3.
Influence of environmental stimuli on the monomeric-to-polymeric ratio of circulating IgA in groups of young (6–8 weeks old) and adult (3–4 months old) mice housed in GF, SOPF, or CIS facilities. (A) Plasma proteins were separated under nonreducing conditions, blotted, and stained with HRP–linked anti–human IgA. (B) Blots from two α1KI and two α1KI J−/− mice were stained with anti–mouse J–chain antiserum. (C) Blots from α1KI J−/− mice housed in SOPF and CIS facilities. (D) Blots from wild-type (WT) mice housed in SOPF and CIS facilities. Molecular mass scale (Mr) is expressed in kilodaltons. Data are representative of multiple experiments (n=4–8).