Figure 7.

In vivo deposited IgA in RAG-2/γc–deficient mice after intraperitoneal grafting of α1KI hybridomas 1, 5, 6, 9, and 10. Each hybridoma was injected in two mice, which were euthanized after tumor development. Ctrl− stands for negative control with ungrafted littermates. (A) Immunofluorescence microscopy on kidney sections stained with FITC–conjugated anti–human IgA (green) and AlexaFluor568-linked antipodocin (red). (B) Quantification of IgA staining by image analysis. Results are means±SEMs of n=10 glomeruli (Kruskall–Wallis test). ***P<0.001. (C) Plasma IgA was measured by ELISA.