Figure 2.

RPA1 depletion induces FANCD2-Ub in the absence of DNA damaging agents. (A) U2OS cells transfected with either control siRNA or RPA1-specific siRNAs were collected at 24, 48 h, and 72h after transfection. Whole cell lysates were analyzed by immunoblotting with indicated antibodies. The FANCD2-Ub: FANCD2 ratios (L: S) are indicated below each sample. (B) U2OS cells were transfected with indicated siRNAs. Whole-cell lysates were prepared 72 hr after transfection and analyzed with indicated antibodies. The FANCD2-Ub: FANCD2 ratios (L: S) are indicated below each sample.