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RPA1 depletion induces higher level of γ-H2AX in FA pathway deficient cells. FANCA deficient (GM6914) and isogenic corrected (GM6814+FANCA) cells were transfected with indicated siRNAs. Whole-cell lysates were prepared 72 hr after transfection and analyzed with indicated antibodies. The FANCD2-Ub: FANCD2 ratios (L: S) are indicated below each sample. Each pair of images was collected using the same exposure time for the comparison from identical gel.
