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. 2016 Jul 15;15(17):2256–2262. doi: 10.1080/15384101.2016.1208872

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© 2016 INSERM - Institut Curie. Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Context-dependent role of Ezh2 in the regulation of the Ink4a/Arf locus. (A) Western blot against Ezh2, H3K27me3 and Lamin B1 (loading control) of iMEF cells immortalized by c-Myc, P53-DN or Ndy1 in the presence or absence of OHT as indicated on top. (B) Top panels, proliferation curves of the 3 iMEF cell lines in the presence or absence of Ezh2 (mean ± SD, n = 3). Bottom panels, RT-qPCR analysis of p16, p19 and p21 genes in the corresponding cell lines. Values indicate relative expression of mutant cells compared to WT cells after normalization to the house keeping gene TATA-binding protein (TBP). ND, not detected. Mean ± SD, n = 3. (C) Snapshot of H3K27me3 ChIP-seq distribution in c-Myc iMEFs (C2 clone) Ezh2 WT or Ezh2 Δ/Δ at the level of the p16/p19 locus (left) and of the p21 locus (right).