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. 2016 Jun 3;17(8):790–798. doi: 10.1080/15384047.2016.1195040

Figure 4.

Figure 4.

HIF- 3α regulates cell proliferation. (A) Clonogenic assay of HeLa cells transfected with NC, miR-147a or siHIF-3α in normoxia. (B) Inhibition of HIF-3α expression inhibits HeLa cell proliferation. HeLa cells transfected with 2 designed HIF-3α siRNAs or NC for 1, 2 or 3 days; viabilities were determined with CCK-8 assay every day. Data shown are mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.001. (C) Transfection with HIF-1α siRNA reduced HIF-1α synthesis in 1% O2and transfection with HIF-1α plasmid increased HIF-1α synthesis in 21% O2 by western blot. (D) Overexpression HIF-1α inhibits cell proliferation in normoxia or hypoxia. HeLa cells were transfected with pcDNA or HIF-1α-pcDNA in 21% O2or 1%O2. Expression of HIF-1α was confirmed by protein gel blot and cell viabilities were determined by CCK-8 assay after 3 d. Data shown are mean ± SD of 3 independent experiments. *P < 0.05. (E) CCK-8 assay of HeLa cells co-transfected with siRNAs or NC; cell viability (OD490 nm absorbance) was examined at 72h after transfection in hypoxia. Data shown are mean ± SD of 3 independent experiments, *P < 0.05, student 2-tailed t test.