Figure 7.
Activation of PAK1 reduced the inhibitory effects of 5-FU on CRC cells. SW480 cells transfected with constitutively active (CA) PAK1 or vector only 26 were cultured with 5-FU. (A) Cell proliferation was determined by cell counting. (B) In the sphere formation assay, VO and CA cells were treated with 5-FU (50 μM), and sphere numbers were counted after 10 d. Spheres containing more than 100 cells were classified as large. L: large, T: total, *, p < 0.05, **, p < 0.01, ***, p < 0.001, compared to the values from VO1 cells (A) or control (cont, B).