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MAb4C5 inhibits colony formation of BCSCs. (A) Anchorage-independent assay using MDA-MB-231cells and mammospheres, in the absence (left panel) or presence (right panel) of mAb4C5. (B) Addition of mAb4C5 resulted in a 73.3% decrease in the number of newly-formed colonies with respect to the controls. (C) quantification of cell viability of all cases studied. Values presented are the mean ± SEM from 3 independent experiments.***p value < 0.0001. Bars correspond to 200 μm.
