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. 2016 Apr 20;15(17):2275–2287. doi: 10.1080/15384101.2016.1172156

Figure 1.

Figure 1.

NUP98 oncoproteins bind the APC/C and prevent MCC binding. A), HEK293 cells were transfected with expression constructs for NUP98, NUP98-HOXD13, NUP98-LOC, NUP98-HHEX (ND13, NLOC, NHHX). Mitotic extracts were obtained from nocodazole-treated cells and subjected to immunoprecipitation (IP) with the indicated antibodies. Anti-IgG (Ig) was used as a negative control. Immunoprecipitated proteins were revealed by immunoblot analysis (WB) with an anti-NUP98 antibody (αNUP98). B), HEK293 were transfected and mitotic extracts were obtained as in A). Samples were immunoprecipitated (IP) with the anti-APC4 antibody (αAPC4). Anti-IgG (Ig) was used as a negative control. Immunoprecipitated proteins were revealed by immunoblotting using the indicated antibodies. C), HEK293 were transfected with expression constructs for Flag-NUP98 or Flag-NUP98-HOXD13, and mitotic extracts were obtained as in A). Immunoprecipitated proteins were revealed by immunoblotting using the indicated antibodies. D), HEK293 were transfected and mitotic extracts were obtained as in A). Immunoprecipitated proteins were revealed by immunoblotting using the indicated antibodies. IgHC, immunoglobulin heavy chains.