Figure 3.

CDC20 and MAD2 rescue SAC attenuation by NUP98 oncoproteins. A), Immunoblots showing securin (SEC) degradation in extracts prepared from Nocodazole-arrested HEK293 cells exogenously expressing the indicated proteins (left). Samples were collected at the indicated times after incubation. Loading control: Anti-ßactin (ßACT). B), HEK293 were transfected with expression constructs for the indicated proteins, mitotic extracts were obtained from nocodazole-treated cells and subjected to immunoprecipitation (IP) with the indicated antibodies. Immunoprecipitated proteins were revealed by immunoblot analysis (WB) with an anti-NUP98 antibody (αNUP98). C), Immunoblots showing securin (SEC) degradation. Mitotic extracts were prepared from nocodazole-arrested HEK293 cells, expressing the indicated proteins (left), as in A).