Figure 2.

Expression pattern of the esyt1 gene in early mouse embryos carrying the β-galactosidase (βGal) allele (Fig. 1A). Expression was determined at stages 11.5, 12.5 and 13.5 dpc by conversion of X-Gal (blue-green), see Materials and Methods. Enlarged panels below the whole mount views of the 13.5 dpc embryos show detail of the lateral mesodermal plate and anterior limb-bud. The images are of embryos homozygous for the βGal insertion allele which lack a functional esyt1 gene. The pattern staining in these embryos was indistinguishable from that of esyt1^+/ßGal mice but was somewhat stronger consistent with a gene dosage effect.