Figure 2.

FUCA1 is induced by gentoxic stress in a p53 dependent manner. (A-F) p53 null HCT116 (−/−p53) and WT HCT116 (+/+p53) (A-B), RKO expressing scrambled (LMP-Scr) or p53-specific (LMP-p53) shRNAs (C-D) and U2OS expressing scrambled (LMP-Scr) or p53-specific (LMP-p53) shRNAs (E-F) were treated with either Adriamycin (Adr)(0.5 μg/ml), ActinomycinD (ActD)(2nM ) or Etoposide (Etop) (20 μm), for 24 hr prior to qRT-PCR quantification of FUCA1 (A,C,E). Data are expressed as relative FUCA1 mRNA level and represented as mean ± SD (n = 2 or 3 independent experiements, 5 to 9 replicates, one way Anova: *p < 0.005 **p < 0.001, p21 ***p < 0.0001). (B,D,F) p53 expression were assessed by western blotting. Immunoblot against actin was used as a loading control.