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. 2016 Jan 21;22:192–201. doi: 10.2119/molmed.2015.00026

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Copyright: © 2016 The Feinstein Institute for Medical Research

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Figure 3. — Synd4 overexpression induced cultured cardiomyocyte hypertrophy. Ad-synd4 was used to activate synd4. Ad-Lacz was used as control. IGF-1 (10 nmol/L) and ISO (10 μm) were added to Ad-Lacz transfected cells to induce typical physiological and pathological cell hypertrophy respectively. (A) Immunofluorescence assay showed increased cell area in synd4-overexpressed, IGF-1-treated and ISO-treated cardiomyocytes. *P < 0.05, Ad-synd4 versus Ad-Lacz, Ad-Lacz + IGF-1 versus Ad-Lacz, Ad-Lacz + ISO versus Ad-Lacz; scale bar = 20 μm; N = 3 experiments, ANOVA. (B) (³⁵S)methionine incorporation assay showed increased protein production in synd4-overexpressed, IGF-1-treated and ISO-treated cardiomyocytes. *P < 0.05, Ad-synd4 versus Ad-Lacz, Ad-Lacz + IGF-1 versus Ad-Lacz, Ad-Lacz + ISO versus Ad-Lacz; N = 3 experiments, ANOVA. (C) Tunel assay showed increase in tunel-positive cells in ISO-treated cardiomyocytes. ^#P < 0.05, Ad-synd4 versus Ad-Lacz + ISO, Ad-Lacz + IGF-1 versus Ad-Lacz + ISO, Ad-Lac versus Ad-Lacz + ISO; scale bar = 50 μm; N = 3 experiments, ANOVA.