Figure 5.

Plumbagin protects mice from endotoxic shock. (A) Mice (n = 20/group) were injected with a single dose of plumbagin (4 mg/kg), followed 30 min later by an infusion of endotoxin (lipopolysaccharide [LPS], 5 mg/kg, intraperitoneally), and were then re-treated with plumbagin 12 and 24 h later. The Kaplan-Meier method was used to compare the differences in survival rates between groups (*, p < 0.05). (B–H) In parallel experiments, serum levels of lactate (B), IL-1β (C), TNF (D), HMGB1 (E), PKM2 mRNA (F), NOX activity (G) and ECAR (H) in isolated peritoneal macrophages at indicated time points were measured (n = 3–5 animals/group, values are mean ± SEM, *, p < 0.05).