Figure 5.

Effect of the combined treatment on liver inflammation in aged septic rats. Rats were sham operated or subjected to CLP with injection of vehicle (normal saline) or GG-2 (80 nmol/kg of ghrelin and 50 μg/kg of GH) at 5 h after CLP. The liver tissues were harvested at 20 h after CLP for measuring (A) protein levels of TNF-α by ELISA, mRNA levels of (B) MIP-2 and (C) KC by qPCR, and (D) MPO activity by colorimetric assay. mRNA levels in sham group are designated 1 for comparison. Data are expressed as mean ± SE (n = 5–7 per group). *P < 0.05 versus sham and ^#P < 0.05 versus vehicle.