Figure 5.

Injected proteinase 3 induces insulin resistance in caspase-1-deficient mice during the last 10 d of a high-fat diet. (A) Bodyweight of caspase-1^–/– mice treated with vehicle (n = 10) or proteinase 3 (n = 10) during last 10 d of a high-fat diet. (B) Fasting glucose levels at baseline. (C) Glucose levels during insulin tolerance test and ITT AUC values. (D) Plasma insulin levels. (E) HE staining of liver sections. (F) Percentage of lipid droplets in HE-stained liver sections, liver weights and liver triglycerides. ITT, insulin tolerance test; PR3, proteinase 3; TG, triglycerides; *significant difference p ≤ 0.05; **significant difference p ≤ 0.01.