Figure 7.

Treatment with human α-1 antitrypsin reduces macrophage infiltration into the adipose tissue during the last 10 d of a high-fat diet. (A) Representative images of immunostaining of the macrophage marker F4/80 in white adipose tissue of low–fat–diet-fed mice treated with vehicle, high–fat–diet-fed mice treated with vehicle and high–fat–diet-fed mice treated with human α-1 antitrypsin. (B) Average number of macrophages per field. (C) Average number of crown-like structures (CLS) per field. Secretion of (D) IL6 and (E) KC by white adipose tissue explants. LFD, low-fat diet; HFD, high-fat diet; hAAT, human α-1 antitrypsin; CLS, crown-like structures; *P ≤ 0.05; NS, nonsignificant.