Figure 3. Coexpression network analysis of genes near differentially methylated CpG dinucleotides in airway epithelial cells from asthmatic and nonasthmatic subjects⁸⁴.

Networks were generated by using Ingenuity Pathway Analysis (IPA), as previously described.⁸⁴ Genes near differentially methylated CpGs are shown as yellow symbols; other genes are included to connect the network of differentially methylated CpGs. Molecular shapes (from IPA) were as follows: oval, transcriptional regulator; diamond, enzyme; dashed line rectangle, channel; upward triangle, phosphatase; downward triangle, kinase; trapezoid, transporter; circle, other. A, A network from module 1 that was associated with clinical markers of asthma severity. This network is centered on extracellular signal-regulated kinase 1/2, which was not itself near a differentially methylated CpG. B, A network from module 2 that was associated with eosinophilia. This network is centered on IFN-g, which was near a differentially methylated CpG. Reprinted with permission of the American Thoracic Society from Nicodemus-Johnson et al.⁸⁴ Copyright 2016 American Thoracic Society.