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Configurations of hydrogen bond network in acylimine region from MD for TagRFP675 (a-c) and for mKate/M41Q (d,e). Percentages were calculated based on the O-O distance between S28 and Q41. The three states in TagRFP675 correspond to Δr<3.5 Å, 3.5Å ≤Δr<4.5 Å and Δr ≥4.5 Å. In mKate/M41Q, the two states correspond to Δr<3.5 Å and Δr ≥3.5 Å.
