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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Cell Signal. 2016 Apr 14;28(8):871–879. doi: 10.1016/j.cellsig.2016.04.006

Figure 5. S1P induces RhoA mediated gene expression through Gα12/13.

Figure 5

Mouse CPCs were transfected with an SRE.L luciferase reporter construct (0.5 µg) for 48 hrs (A) Cells were stimulated with S1P (µM) or 20% serum for 6 hours with or without pre-treatment with C3 toxin (1ug/ml). #p≤0.05 vs. non-treated (NT); *p≤0.05 vs. agonist alone by one-way ANOVA; n=12, 4 independent experiments (B) Cells were transfected with siControl or siRNA targeting various G protein α subunits. Knockdown of G protein mRNAs assessed by qPCR was ~80% for Gα12, ~100% for Gα13, ~90% for Gαq and Gαi . SRE.L luciferase activity was normalized to Renilla and expressed as percentage of maximal S1P induction with control siRNA. ***p≤0.005 vs. Max S1P response (100%) by unpaired t-test; n=4 independent experiments.