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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Cell Signal. 2016 Apr 14;28(8):871–879. doi: 10.1016/j.cellsig.2016.04.006

Figure 6. S1P induces cardiac lineage gene expression in a MRTF-A dependent manner.

Figure 6

Starved mouse CPCs were treated with S1P (3µM) for 3 days and Mef2C, GATA4 and GATA6 expression levels were measured by qPCR. Fold changes over control at time 0 are graphed. Pharmacological inhibition (10µM, CCG-293971) (A) or silencing (B) of MRTF-A attenuate the effect of S1P on cardiac gene expression. *p≤0.05, **p≤0.01, ***p≤0.005 vs. S1P treated by unpaired t-test; n=8, 3 independent experiments. (C) CPCs were transfected with a plasmid encoding MRTF-A (0.5 µg). Overexpression of MRTF-A for 48 hours induced cardiac gene expression in CPCs. n=12, 4 independent experiments. *p≤0.05 and ***p≤0.005 vs. control at time 0 by unpaired T-test. (D) qPCR analysis of gene expression in S1PR KO CPCs following 3 days of S1P stimulation *p≤0.05 **p≤0.01 vs. WT S1P treated by unpaired t-test; n = 9, 3 independent experiments