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Effects of Pyk2 RNAi on early embryonic development of mice. Pyk2 siRNA and negative control siRNA was microinjected into fertilized eggs. A: interference efficiency was determined by RT-qPCR. Pyk2 mRNA levels in zygotes after RNAi was 18.9% relative to the negative control. B: Embryonic development after Pyk2 RNAi. The 2-, 4-, and 8-cell rates determined using an independent sample T-test; no significant difference was found between the siRNA and the negative control groups.
