Table 3. Developmental competence of in vitro grown bovine oocytes after in vitro maturation and fertilization.
| Growth 1 | Insemination (h) | No. of oocytes used 2
(replicates) |
No. of blastocysts (%) 3 |
|---|---|---|---|
| In vitro | 6 | 95 (7) | 1 (1)a |
| 12 | 98 (7) | 22 (22)b | |
| In vivo | 6 | 136 (6) | 31 (23)b |
| 12 | 126 (6) | 28 (22)b |
1 OGCs collected from 0.4−0.7 mm antral follicles were subjected to in vitro growth culture for 14 days followed by in vitro maturation and fertilization (In vitro). Examined oocytes from 4−6 mm antral follicles were used as in vivo controls (In vivo). 2 Number of oocytes used for in vitro fertilization. 3 After in vitro fertilization, oocytes were further cultured for development up to 8 days. a, b Values with different superscripts in the same column differ significantly (P < 0.05).