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. 2016 Aug 30;12(8):e1005841. doi: 10.1371/journal.ppat.1005841

Fig 1. Interaction network of DENV NS5 and human proteins from infected cells.

Fig 1

(A) Construction of recombinant DENVs. Viruses with Strep-tag fused to NS5 in different positions: N-terminus (r1-DV), inter-domain linker (r2-DV), low conserved loop region (r3-DV) and C-terminus (r4-DV) are shown. Immunofluorescence assays after RNA transfection using specific anti-E antibodies show replication of each virus. (B) Flow chart of experimental procedure for NS5-containing protein complex purification. Human cells (HEK 293T or Huh7) were mock infected, DENV WT infected, or DENV NS5-tag infected in parallel. (C) Coomassie blue staining showing the recovery of NS5 binding partners. (D) Results of the mass spectrometry analysis, the major functional categories of host proteins identified as NS5 interactors are depicted. (E) NS5-host proteins interaction network. Lines depict interactions detected in this study between NS5 and human proteins. Also, interaction between human proteins extracted from database (http://string-db.org) is shown. Components of the spliceosomal U5 snRNP are shown in red. Previously reported NS5 interactors are indicated in green.