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. 2016 Aug 30;12(8):e1005841. doi: 10.1371/journal.ppat.1005841

Fig 4. NS5 alone interferes with alternative splicing.

Fig 4

(A) NS5 shows a dose dependent effect on the splicing reporter minigenes CFTR, EDI, and Bclx. Radiolabeled amplification products corresponding to different splicing isoforms derived from the indicated minigenes are shown. (B) Quantitative analysis of the changes induced by NS5 (0.9 μg of plasmid). (C) Expression of NS5 mutants with impaired methyltransferase (Mut-MTase) or polymerase (Mut-RdRp) enzymatic activity also alters alternative splicing of reporter genes.