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. 2016 Aug 30;12(8):e1005841. doi: 10.1371/journal.ppat.1005841

Fig 7. NS5 reduces the splicing efficiency of endogenous RIG-I mRNA.

Fig 7

(A) Schematic representation of RIG-I pre-mRNA and mRNA. The abundance of these forms was measured by quantifying the immature and mature RNA transcripts using the region corresponding to exon 10, exon 11 and the intron between them, as indicated. The relative positions of the primers designed for RT-qPCR are depicted. (B) NS5 expressed as a mature protein significantly reduces the RIG-I splicing efficiency compared with GFP or an empty vector control. Data corresponding to four independent experiments is shown, mean ± SD. (C) RIG-I splicing efficiency (estimated as the RIG-I mRNA/pre-mRNA ratio) was not altered by α-IFN. (D) NS5 expressed as a mature protein does not induce STAT2 protein degradation.