Table 1. Primer sets and restriction enzymes used to genotype 8 tag SNPs.
| Descriptiona | dbSNP accession numberb | Position in VLDLR | Primer sequences | Restriction enzymes |
|---|---|---|---|---|
| g.26 419 086 G > A | rs312633123 | Intron 1 | F: 5'-AAAGCCTGGATAAGAGCG-3' | HaeIII |
| R: 5'-TTCCAAATGAAGGGAAGC-3' | ||||
| g.26,420,908T > G | novel SNP | Intron 2 | F: 5'-TAACAGCAGGAAGTGGAT-3' | HinfI |
| R: 5'-GCATACTAATGGCAACAA-3' | ||||
| g.26,423,124A > G | novel SNP | Intron 4 | F: 5'-TCAGGGTTTCTTAACAGC-3' | SnaBI |
| R: 5'-ACACCTCAGGCAACTCAT-3' | ||||
| g.26,424,657G > A | rs317219970 | Exon 6 (synonymous mutation) | F: 5'-GATGAAATCAACTGCCGTAA-3' | XbaI |
| R: 5'-CAGGTCCAGAACACTGAATAAC-3' | ||||
| g.26426398T > C | rs317314828 | Intron 10 | F: 5'- GCCAAATCTGTATCAACC-3' | MfeI |
| R: 5'- ACGGGTATCAATAGAGGC-3' | ||||
| g.26,427,922AG | rs314567990 | Intron 14 | F: 5'-CGTGTATTCTGGATTGACGGAG-3' | HinfI |
| R: 5'-CAGCAGGGGTGCTAGGCT-3' | ||||
| g.26,429,604A > C | rs14777635 | Intron 15 | F: 5'-ATGAAATGCCAAGAGTGC-3' | ApoI |
| R: 5'-AAGTAGTGAGGCTGCTTA-3' | ||||
| g.26,431,186C > T | rs314284862 | 3' UTR | F: 5'-ACGGGTTACTATGATTGC-3' | BseRI |
| R: 5'-TCCAGCCAGACTCTTACA-3' |
a
SNP positions on chromosome Z are based on the chicken genome galGal4 assembly.
b
Accession number in the dbSNP database (http://www.ncbi.nlm.nih.gov/snp)