Figure 1. Carbonic anhydrases transcripts patterns in Paracoccididoides. (A) Transcripts patterns for carbonic anhydrases in phases of Paracoccidioides. Yeast cells were grown at 36 °C and mycelium at 22 °C. Mycelium was induced to undergo morphological differentiation for 22 and 48 hours, by changing the incubation temperature to 36 °C. The values of fold change are normalized to the mycelia phase. (B) Transcripts patterns in yeast cells incubated with 5% CO₂. Yeast cells of Paracoccidioides upon incubation at 36 °C, in the presence of 5% CO₂ at 2, 6, 24 and 48 hours. The values of fold change are normalized to the yeast cells growing in vitro. (C) Carbonic anhydrases transcripts patterns in yeast cells recovered from mouse liver and spleen. Expression levels of transcripts from yeast cells derived from liver and spleen of mouse after 7 and 15 days of infection. The values of fold change are normalized to yeast cells growing in vitro. Data of the three panels were normalized against Paracoccidioides alpha tubulin mRNA level. Bars indicate the standard deviation of biological triplicates and asterisks denote values statistically significant (p ≤ 0.05).