Figure 3. Effects of pH and temperature on stability of purified recombinant carbonic anhydrases. (A) The enzymes rCA1 and rCA4 were incubated in citrate-phosphate buffer in different pHs for 2 hours at room temperature. The residual activity was measured according to the standard enzyme assay. (B) Effect of temperature on stability of purified carbonic anhydrase (rCA1 and rCA4). The reaction mixture containing purified enzyme was incubated at different temperatures between 30 °C at 60 °C for 1 hour. The residual enzyme activity was determined.
