Fig 7. Ferritin and iron deposition in the kidney and spleen of DFP-treated mice.

Histological and immunohistochemical studies of DFP-treated and control FTL-Tg mice (A-F). Analysis of paraffin embedded sections from control (A, D), DFP₅₀ (B, E), and DFP₁₀₀ (C, F) treated FTL-Tg mice. Sections shown are from kidney (A-C) and spleen (D-F). Sections were immunostained with Abs against the mutant L chain (A-C) or stained with the Perls’ Prussian blue method (D-F). Original magnifications x10 (D-F), x40 (A-C). Western blot analysis of protein samples from the kidney using antibodies specific for the L, Lm, and H chains (B). β-actin was used as loading control. Representative blots are shown for five control, three DFP₅₀, and four DFP₁₀₀ male mice. Densitometric analysis from three independent experiments shows statistical significant differences between the controls and DFP-treated mice in the supernatant (G) and the pellet (H). (*p < 0.05). By the colorimetric ferrozine method, a decrease in the levels of non-heme iron in the kidney of DFP-treated FTL-Tg mice compared with non-treated FTL-Tg controls was observed in the supernatant (DFP₅₀, p = 0.0190; DFP₁₀₀, p = 0.0299) (I) and the pellet (DFP₁₀₀, p = 0.0032) (J).