Table 2. Experimental conditions used in each experiment performed at different temperatures and nutrient enrichment.
Experiment | Nutrient enrichment | Temperature (°C) | Treatment volume (ml) | Replicates | Exposure time | Stage exposed | Number of individuals per replicate | Variable measured |
---|---|---|---|---|---|---|---|---|
1a | Low, medium, high | 27, 29, 30, 31, 32 | 12 | 6 | 2.5 hours | Gametes | ~170 eggs | % Fertilization |
1b | Low, medium, high | 27, 29, 30, 31, 32 | 12 | 6 | 2.5 hours | Gametes | % Abnormalities | |
1c | Low, medium, high | 27, 29, 30, 31, 32 | 40 | 6 | 5 days | Larvae | 20 larvae | % Larvae survivorship |
1d | Low, medium, high | 27, 29, 30, 31, 32 | 10 | 6 | 6 days | Larvae | 10 larvae | % Larvae settlement |
2 | Low, medium, high | 27, 29, 30, 31, 32 | 10 | 12 | 1 day | Larvae | 10 larvae | % Larvae settlement |
3 | Low, medium, high | 27, 30, 32 | 4000 | 2 | 59 days | Juveniles | 19 juveniles | Growth, production of new polyps, final weight Fv/Fm, survivorship |