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. 2016 Aug 30;11(8):e0161993. doi: 10.1371/journal.pone.0161993

Table 1. Primer sequences for quantitative reverse transcription-PCR.

Gene Sequence
ALDH2 Sense 5’- TTATCCAGCCCACCGTGTTC-3’
Anti-sense 5’- GCTGCCAGCCCATACTTAGA-3’
FABP4 Sense 5’- GATGCCTTTGTGGGAACCTG-3’
Anti-sense 5’- GCCATGCCTGCCACTTTC-3’
ADIPONECTIN Sense 5’- TCCTGGAGAGAAGGGAGAGAAAG-3’
Anti-sense 5’- CCCTTCAGCTCCTGTCATTCC-3’
LPL Sense 5’-GGATGGACGGTAAGAGTGATTC-3’
Anti-sense 5’-ATCCAAGGGTAGCAGACAGGT-3’
ACC Sense 5’-AACATCCCCACGCTAAACAG-3’
Anti-sense 5’-CTGACAAGGTGGCGTGAAG-3’
PPARγ Sense 5’-CAAGAATACCAAAGTGCGATCAA-3’
Anti-sense 5’-GAGCTGGGTCTTTTCAGAATAATAAG-5’
C/EBPα Sense 5’-GAACAGCAACGAGTACCGGGTA-3’
Anti-sense 5’-GCCATGGCCTTGACCAAGGAG-3’
C/EBPβ Sense 5’-CAAGCTGAGCGACGAGTACA-3’
Anti-sense 5’-CAGCTGCTCCACCTTCTTCT-3’
AQP7 Sense 5’-AGGCATTCGTGACTGGGATG-3’
Anti-sense 5’-CACCCCAAGGACGGTAACAA-3’
ACOX2 Sense 5’-CTTGGCATGTTGGTGACACG-3’
Anti-sense 5’-TCACTAGGCCGAAGACGAGA-3’
PCK1 Sense 5’-TGCCTCCTCAGCTGCATAAC-3’
Anti-sense 5’-GGATATACTCCGGCTGGCAC-3’
NR1H3 Sense 5’-GGGATAGGGTTGGAGTCAGC-3’
Anti-sense 5’-GGCCCTTTTTCCGCTTTTGT-3’
OLR1 Sense 5’-CATCCTCTGCCTGGTGTTGT-3’
Anti-sense 5’-TCTGCCCTTCCAGGATACGA-3’
PREF-1 Sense 5’-GCGTGGACCTGGAGAAAG-3’
Anti-sense 5’-GGAAGTCACCCCCGATGT-3’
18S Sense 5’- ACGATGCCGACTGGCGATGC-3’
Anti-sense 5’- TCCTGGTGGTGCCCTTCCGT-3’

Abbreviations used: aldehyde dehydrogenase 2, ALDH2; adipocyte fatty acid binding protein, FABP4; lipoprotein lipase, LPL; acetyl-CoA carboxylase, ACC; peroxisome proliferator-activated receptor γ, PPARγ; CCAAT-enhancer-binding protein α, C/EBPα; CCAAT-enhancer-binding protein β, C/EBPβ; aquaporin 7, AQP7; acyl-Coenzyme A oxidase 2, branched chain, ACOX2; phosphoenolpyruvate carboxykinase 1 (soluble), PCK1; nuclear receptor subfamily 1, group H, member 3, NR1H3; oxidised low density lipoprotein (lectin-like) receptor 1, OLR1.