Table 1. Primer sequences for quantitative reverse transcription-PCR.
Gene | Sequence | |
---|---|---|
ALDH2 | Sense | 5’- TTATCCAGCCCACCGTGTTC-3’ |
Anti-sense | 5’- GCTGCCAGCCCATACTTAGA-3’ | |
FABP4 | Sense | 5’- GATGCCTTTGTGGGAACCTG-3’ |
Anti-sense | 5’- GCCATGCCTGCCACTTTC-3’ | |
ADIPONECTIN | Sense | 5’- TCCTGGAGAGAAGGGAGAGAAAG-3’ |
Anti-sense | 5’- CCCTTCAGCTCCTGTCATTCC-3’ | |
LPL | Sense | 5’-GGATGGACGGTAAGAGTGATTC-3’ |
Anti-sense | 5’-ATCCAAGGGTAGCAGACAGGT-3’ | |
ACC | Sense | 5’-AACATCCCCACGCTAAACAG-3’ |
Anti-sense | 5’-CTGACAAGGTGGCGTGAAG-3’ | |
PPARγ | Sense | 5’-CAAGAATACCAAAGTGCGATCAA-3’ |
Anti-sense | 5’-GAGCTGGGTCTTTTCAGAATAATAAG-5’ | |
C/EBPα | Sense | 5’-GAACAGCAACGAGTACCGGGTA-3’ |
Anti-sense | 5’-GCCATGGCCTTGACCAAGGAG-3’ | |
C/EBPβ | Sense | 5’-CAAGCTGAGCGACGAGTACA-3’ |
Anti-sense | 5’-CAGCTGCTCCACCTTCTTCT-3’ | |
AQP7 | Sense | 5’-AGGCATTCGTGACTGGGATG-3’ |
Anti-sense | 5’-CACCCCAAGGACGGTAACAA-3’ | |
ACOX2 | Sense | 5’-CTTGGCATGTTGGTGACACG-3’ |
Anti-sense | 5’-TCACTAGGCCGAAGACGAGA-3’ | |
PCK1 | Sense | 5’-TGCCTCCTCAGCTGCATAAC-3’ |
Anti-sense | 5’-GGATATACTCCGGCTGGCAC-3’ | |
NR1H3 | Sense | 5’-GGGATAGGGTTGGAGTCAGC-3’ |
Anti-sense | 5’-GGCCCTTTTTCCGCTTTTGT-3’ | |
OLR1 | Sense | 5’-CATCCTCTGCCTGGTGTTGT-3’ |
Anti-sense | 5’-TCTGCCCTTCCAGGATACGA-3’ | |
PREF-1 | Sense | 5’-GCGTGGACCTGGAGAAAG-3’ |
Anti-sense | 5’-GGAAGTCACCCCCGATGT-3’ | |
18S | Sense | 5’- ACGATGCCGACTGGCGATGC-3’ |
Anti-sense | 5’- TCCTGGTGGTGCCCTTCCGT-3’ |
Abbreviations used: aldehyde dehydrogenase 2, ALDH2; adipocyte fatty acid binding protein, FABP4; lipoprotein lipase, LPL; acetyl-CoA carboxylase, ACC; peroxisome proliferator-activated receptor γ, PPARγ; CCAAT-enhancer-binding protein α, C/EBPα; CCAAT-enhancer-binding protein β, C/EBPβ; aquaporin 7, AQP7; acyl-Coenzyme A oxidase 2, branched chain, ACOX2; phosphoenolpyruvate carboxykinase 1 (soluble), PCK1; nuclear receptor subfamily 1, group H, member 3, NR1H3; oxidised low density lipoprotein (lectin-like) receptor 1, OLR1.