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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Microbiol. 2015 Oct 20;99(1):135–150. doi: 10.1111/mmi.13221

Figure 3. Both Borrelia burgdorferi p66 and HtrA partition primarily into detergent resistant membranes (DRM). B. burgdorferi were treated overnight with 1%Triton X-100 at 4°C and the lysate was separated on a discontinuous OptiPrep density gradient. Fractions containing 20%, 25%, 30% and 35% OptiPrep were collected and analyzed by slot blot.

Figure 3

(A) Rabbit anti-α-asialo GM1 purified IgG was used to identify fractions containing DRM.

(B) Rabbit anti-p66 immune serum was used to indicate the presence of p66 protein in DRM.

(C) Rabbit anti-HtrA immune serum was used to indicate the presence of HtrA protein in DRM.

(D) Mouse anti-DnaK was used to indicate the absence of DnaK in DRM and preference for the soluble fraction.

(E) Rabbit anti-OspC was used to indicate the absence of OspC in DRM and preference for the soluble fraction.