Fig 2. IFN-γ causes ROS generation and NADPH oxidase expression.

(A) ROS generation in IFN-γ (10 ng/ml)-treated A549 cells was determined by CM-H₂DCFDA staining followed by analysis using a fluorescent plate reader for the indicated time. The data are presented as the mean ± SD of triplicate cultures and are shown as the relative optical densities (O.D.). *P < 0.05 and **P < 0.01. PBS was used as a control. (B) Representative Western blotting shows gp91^phox, p67^phox, and p47^phox expression in IFN-γ (10 ng/ml)-treated A549 cells for the indicated time. β-actin was used as an internal control. The relative ratios of the measured proteins and β-actin are also shown as the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01. ns, not significant.