Skip to main content
. 2016 Aug 30;11(8):e0162157. doi: 10.1371/journal.pone.0162157

Fig 5. p47phox is key for IFN-γ-induced DNA damage and histone H3 citrullination.

Fig 5

(A) After IFN-γ (10 ng/ml) treatment in shLuc- or shp47phox-transfected A549 cells for 6 days, immunostaining with fluorescence microscopy was performed to determine the percentages of phosphor-γ-H2AX (Ser139)-positive nuclei. The data are shown as the mean ± SD of three independent experiments. ***P < 0.001. (B) After IFN-γ (10 ng/ml) treatment in shLuc- and shp47phox-transfected A549 cells for 6 days, Western blotting was performed to detect citrullinated histone H3 (Cit. H3) and histone H3. β-actin was used as an internal control. The relative ratios of Cit. H3 and β-actin are also shown as the mean ± SD of three independent experiments. ***P < 0.001.