Fig. 8.

LGN loss does not affect hair follicle development. (A) Inverted images of K14 (basal marker) in wild-type and LGN knockdown E17.5 backskin, where green lines indicate hair follicle (HF) angling. (B) Radial histograms show HF orientation in wild type and LGN knockdowns (mean indicated with green lines; P=0.5307 by Mann–Whitney test). (C,D) Images (C) and quantification (D) of HFs at each stage in LGN knockdowns and wild-type littermates. (E-H) Characteristic suprabasal Sox9⁺ cells (arrowheads) in hair placodes (E) and in Pcad⁻ regions of hair pegs (F), as quantified in G,H. P-values determined by two-tailed Student's t-test (D), binomial test (G) or Mann–Whitney test (H). Scale bars: 100 µm in A; 50 µm in C; 10 µm in E; 25 µm in F.