Skip to main content
. 2016 Aug 15;126(9):3433–3446. doi: 10.1172/JCI86522

Figure 5. Short-term inducible deletion of IR and IGF1R in muscle induces atrophy, autophagy, and FoxO target gene expression.

Figure 5

(A) Western blot analysis of IR and IGF1R in TA muscle from control and MindIGIRKO (muscle inducible IGF1R IR knockout) mice sacrificed at various times before or after a 6-day treatment of tamoxifen. Day 0 is the last day of tamoxifen injection. (B) Western blots for autophagy intermediates in TA muscle from control or MindIGIRKO mice 21 days after the last injection of tamoxifen. (C) Dissected muscle weights at day 21 in control or MindIGIRKO mice. (D) qPCR for mRNA levels of IR, Igf1r, FoxO target genes, autophagy genes, proteasome subunit genes, and ubiquitin ligase genes in TA muscle at day 21 in control or MindIGIRKO mice. (n= 5-6). (*P < 0.05, **P < 0.01 vs. day 21 control, Student’s t test.)