Figure 1. Oxidative changes in lateral line hair cells following aminoglycoside exposure monitored with cellROX.

(A) Transformed (F/F₀) fluorescence intensity data of individual cells loaded with cellROX that die following exposure to 50 μM neomycin. Colors indicate individual hair cells. (B) Transformed (F/F₀) fluorescence intensity data of individual cellROX-labeled cells that survive following exposure to 50 μM neomycin. The dotted line in A and B represents (F/F₀) of 1, corresponding to preneomycin baseline levels. (C) Scatter plot of maximal cellROX fluorescence change in surviving or dying cells exposed to 50 μM neomycin. Horizontal line and error bar represent the mean ± 1 SEM. One-way ANOVA, Holm-Šidák multiple comparison post-test; **P < 0.01, ***P < 0.001. Individual points in C are mean neuromast fluorescence taken from fewer than 5 cells per neuromast and 1 to 3 neuromasts per animal. n = 15 (control), 11 (xanthine), 13 (neomycin, living), and 13 (neomycin, dying) neuromasts. Data from neomycin experiments (living versus dying) are paired data.