Skip to main content
. 2016 Aug 8;126(9):3556–3566. doi: 10.1172/JCI84939

Figure 3. Timing of cytoplasmic oxidation (HyPer) relative to mitochondrial membrane potential (TMRE) in dying hair cells exposed to aminoglycosides.

Figure 3

(A) Mean HyPer fluorescence in dying lateral line hair cells colabeled with TMRE and exposed to 400 μM neomycin. Data are aligned to TMREhalf-min, corresponding to dye redistribution from mitochondria into cytoplasm. (B) Mean cross-correlation values of HyPer relative to TMRE. An offset of t = 0 indicates that the events are inseparable. In this case, maximal cross-correlation value (R) was at 0 minutes (r = 0.401, P = 0.011), indicating that the initial onset of increased HyPer fluorescence coincides with the onset of increased TMRE fluorescence. Error bars = SEM; n = 39 cells from 1 to 3 neuromasts per animal and 5 animals.