Figure 5. Cytoplasmic oxidation (HyPer) is correlated with mitochondrial Ca²⁺ uptake (mitoRGECO) during aminoglycoside-induced hair cell death.

(A) Mean transformed (F/F₀) intensity of HyPer and mitoRGECO within dying hair cells exposed to 400 μM neomycin aligned to the time at which they are cleared from the neuromast. The dashed line represents (F/F₀) of 1, corresponding to preneomycin baseline levels. (B) Mean cross-correlation of transformed fluorescence intensity values of HyPer relative to mitoRGECO events occurring within the same cell. Maximal cross-correlation value (R) was at –1 minute (r = 0.411, P = 0.013), indicating that the initial onset of increased mitoRGECO fluorescence (mitochondrial Ca²⁺ uptake) precedes HyPer by 1 minute. Error bars = ±1 SEM; n = 36 cells from 1 to 3 neuromasts per animal and 7 animals.